Effect of Various Culture Parameters on the Bio-surfactant Production from Bacterial Isolates

Biosurfactants are those compounds which contains both hydrophilic and hydrophobic moieties i.e., amphiphilic in nature. They have advantages over the chemical surfactant such as low toxicity, eco-friendly, etc. They possess wide range of industrial applications such as bioremediation, food processing, oil industries and health care. Various microorganisms have the ability to produce the potential biosurfactant. In the present study, microbial culture which showed highest biosurfactant activity i.e., SA3 based on the oil displacement activity and emulsion layer was selected for various culture parameters optimization. Sodium nitrate as a nitrogen source and dextrose as a carbon gives the highest biomass and biosurfactant production. Further 2% of dextrose and 2% of inoculum size provides the highest biomass and biosurfactant production. Growth kinetics and biosurfactant kinetics study reveals that the maximum biomass was produced after 96 h and maximum production of biosurfactant was after 72 h of incubation period. Overall, the results indicated the potential use of SA3 isolate in bioremediation processes.